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目的探讨突触素I(synapsin I)在丙烯酰胺(acrylamide,ACR)大鼠亚急性神经损伤中的变化。方法 48只Wistar大鼠,雄雌各半,腹腔注射ACR染毒(7.5、15和30 mg/kg ACR),生理盐水(normal saline,NS)对照;步态评分评价神经行为改变;电子显微镜检测脊髓神经突触的改变;免疫组化法检测synapsin I的定性、定位改变,Western-blot检测synapsin I的定量变化。结果染毒结束时,30 mg/kg ACR染毒组大鼠体重较对照显著减轻,突触数量较对照显著减少,步态评分较对照显著增加(P<0.05);对照组和ACR各剂量染毒组synapsin I的定位一致,均位于脊髓背角灰质;对照组脊髓背角灰质神经纤维末梢synapsin I呈强阳性反应,随染毒剂量增加该阳性信号减弱;30 mg/kg ACR组synapsin I蛋白表达显著降低(P<0.05)。结论 synapsin I在ACR亚急性神经损伤中的变化显著,与神经行为及神经病理学变化相符,可能是ACR神经突触损伤的作用靶点之一。
Objective To investigate the changes of synapsin I in subacute nerve damage in acrylamide (ACR) rats. Methods 48 Wistar rats were divided into two groups: male and female rats. ACR (7.5, 15 and 30 mg / kg ACR) and normal saline (NS) Spinal cord synapse changes; immunohistochemistry synapsin I qualitative, localization changes, Western-blot detection of synapsin I quantitative changes. Results At the end of exposure, the body weight of rats in 30 mg / kg ACR group was significantly lower than that in control group, the number of synapses was significantly decreased compared with control group, and the score of gait group was significantly higher than that of control group (P <0.05) Synapsin I in the poisoning group was located in the dorsal horn of spinal cord. The synapsin I in the dorsal horn of spinal cord in the control group was strongly positive. The positive signal was attenuated with the increase of the dose of synapsin I. The synapsin I protein The expression was significantly decreased (P <0.05). Conclusions synapsin I has significant changes in ACR subacute nerve injury, which is consistent with neurobehavioral and neuropathological changes and may be one of the targets of synaptic ACR injury.