本文介绍了以收集~3H-TdR前标记靶细胞为指标的检测巨噬细胞细胞毒活性的方法,并对其条件进行了分析。选用小鼠腹腔渗出性巨噬细胞,经24小时激活后,与~3H-TdR标记好的靶细胞共育24小时,收集细胞,测定细胞中残存放射量。从放射量(cpm)的减少来计算杀伤活性。实验表明,衣法结果准确、稳定、简便易行,而且无自发释放和~3H-TdR再利用问题。 This article describes a method for detecting macrophage cytotoxicity by collecting target cells labeled with ~3H-TdR, and analyzes the conditions. The mouse peritoneal exudative macrophages were selected and incubated with 24 h, and were incubated with ~3H-TdR-labeled target cells for 24 hours. The cells were collected and the residual amount of cells in the cells was measured. The killing activity was calculated from the reduction in the amount of radiation (cpm). Experiments show that the results of the clothing method are accurate, stable, and simple, and there is no spontaneous release and ~3H-TdR reuse problem.