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目的通过测定并对比白头翁、兴安白头翁、朝鲜白头翁和细叶白头翁4种白头翁属植物的Internal Transcribed Spacer(ITS)序列,为白头翁属植物的分子鉴定和遗传多样性研究提供依据。方法提取4种白头翁属植物的DNA,扩增ITS序列并克隆测序。利用Clustal X、DNAMAN、MEGA 5等软件分析白头翁属植物的ITS序列特征。结果建立了4种白头翁植物ITS全序列数据库,包括14个变异位点和4个信息位点。细叶白头翁与朝鲜白头翁之间的遗传距离最小,与兴安白头翁遗传距离最大。结论 4种白头翁属药用植物的ITS序列可为其分子鉴定和遗传多样性研究提供依据。
Objective To determine and compare the Internal Transcribed Spacer (ITS) sequences of four species of Pulsatilla (Pulsatilla, Xingan Pulsatilla, Pulsatilla and Pulsatilla) in Pulsatilla, providing evidence for the molecular identification and genetic diversity of Pulsatilla. Methods DNA from four species of Pulsatilla was extracted, ITS sequences were amplified and sequenced. ITS sequences of Pulsatilla were analyzed by software such as Clustal X, DNAMAN and MEGA 5. Results Four full-length databases of Pulsatilla ITS were constructed, including 14 variant sites and 4 information sites. The smallest genetic distance between Pulsatilla and Pulsatilla occidentalis and the largest genetic distance between Pulsatilla and Xingan Pulsatilla. CONCLUSION: ITS sequences of 4 species of Pulsatilla in medicinal plants may provide evidence for their molecular identification and genetic diversity.