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大蒜和木贼提取物以及生物洁净剂和香茅油被用来测定对马铃薯晚疫病菌的孢子萌发、菌丝生长以及对叶片侵染的抑制作用 ,百菌清和清水用作对照。生物洁净剂和大蒜提取物对晚疫病菌孢子萌发具有最好的抑制作用。随着提取物浓度的提高 ,抑制效果也相应提高直到抑制率 10 0 %。生物洁净剂和大蒜提取物的所有浓度处理均完全抑制了游动孢子的萌发。木贼提取物也显示了抑菌特性 ,但程度较低。香茅油对孢子囊的萌发未显示抑制作用但对游动孢子的萌发有一定抑制作用。新鲜大蒜提取物比半干燥大蒜提取物有更强的抑菌作用。新制备的提取物与在 4℃条件下放置 1周的提取物相比在抑菌作用上没有显著差异 ,然而 ,来自新鲜木贼茎叶的提取物比干燥茎叶的提取物对游动孢子萌发抑制作用更强。无论经过灭菌与否 ,0 .12 5 %的生物洁净剂能完全抑制晚疫菌菌落的形成 ,说明它对高温不敏感。大蒜提取物在经高温处理后抑菌作用显著下降。对菌丝生长抑制效果从高到低排列依次为 :生物洁净剂 ,大蒜 ,木贼和香茅油。所有提取物和天然产物对马铃薯叶片上的病菌不表现治疗作用。0 12 5 %的生物洁净剂在接种前 3d和接种同时都显著地抑制了病原菌的侵染 ,其效果等同于百菌清。 2 %的大蒜提取物在接种前 1d和接种同时对病菌侵染的抑制率
Garlic and Equisetum extract, as well as bio-cleaner and citronella oil, were used to determine the inhibition of spore germination, mycelial growth, and leaf infestation of P. infestans, and chlorothalonil and fresh water were used as controls. Biological detergents and garlic extracts have the best inhibitory effect on spore germination of Phytophthora infestans. With the increase of extract concentration, the inhibitory effect is also increased until the inhibition rate of 10%. All concentrations of biocide and garlic extract completely inhibited zoospore germination. Equisetum extract also shows antibacterial properties, but to a lesser extent. Citronella oil on the germination of sporangia did not show inhibition of the germination of zoospores have a certain inhibitory effect. Fresh garlic extract has stronger antibacterial effect than semi-dried garlic extract. There was no significant difference in antibacterial effect between the freshly prepared extract and the extract that had been left standing for 1 week at 4 ° C. However, the extracts from the fresh stems and leaves of stems and leaves were more effective than the extracts of dried stem and leaves against zoospores Germination inhibition stronger. Whether sterilized or not, 0.12 5% of biological detergents can completely inhibit the formation of P. infestans colonies, indicating that it is insensitive to high temperatures. Antibacterial effect of garlic extract decreased significantly after heat treatment. On the mycelial growth inhibitory effect ranked from high to low: bio-cleaner, garlic, Equisetum and citronella oil. All extracts and natural products show no therapeutic effect on germs on potato leaves. 0 12 5% of biological detergents before inoculation 3d and vaccination at the same time significantly inhibited the infection of pathogens, the effect is equivalent to chlorothalonil. The inhibitory rate of 2% garlic extract on pathogen infection 1d before inoculation and inoculation