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目的利用胰腺癌患者血清筛选睾丸 cDNA 噬菌体表达文库,寻找胰腺癌特异性抗原,尤其是肿瘤-睾丸抗原。方法采用血清学筛选重组 cDNA 表达文库(SEREX)技术筛选睾丸cDNA 噬菌体表达文库。对获得的阳性克隆片段进行测序、鉴定和同源性比较。检测40例胰腺导管腺癌患者和40名健康对照血清中抗阳性克隆的抗体。结果筛选共获得107个阳性克隆,代表14个不同抗原基因,其中有13个基因与基因库中已知基因有很高的同源性,这些基因为 activin 受体AⅡ、LOC92912、KLHL12、IFI16和 CAGE 等。另有1个阳性克隆 HS1在基因库和 SEREX 数据库中均未发现有同源基因。胰腺癌患者组 HS1和 HS2克隆抗体阳性率显著高于健康对照组(分别15%与0%比较,X~2=4.50,P<0.05;22.5%与5%比较,X~2=5.16,P<0.05)。结论 HS1可能是1个新的肿瘤一睾丸抗原基因。有必要进一步研究这些阳性克隆的生物学功能和临床价值。
Objective To screen the cDNA library of testis cDNA phages by using serum from patients with pancreatic cancer and to find pancreatic cancer-specific antigen, especially tumor-testis antigen. Methods Sertoli cell cDNA phage expression library was screened by serological screening of recombinant cDNA expression library (SEREX). The positive clones obtained were sequenced, identified and homologous comparison. The anti-positive clones were detected in 40 patients with pancreatic ductal adenocarcinoma and 40 healthy controls. Results A total of 107 positive clones were screened, representing 14 different antigen genes. Among them, 13 genes were highly homologous to known genes in the gene bank. These genes were activin receptor AⅡ, LOC92912, KLHL12, IFI16 and CAGE and so on. In addition, one positive clone, HS1, showed no homologous genes in both the gene bank and the SEREX database. The positive rates of HS1 and HS2 in patients with pancreatic cancer were significantly higher than those in healthy controls (15% vs 0%, respectively, X ~ 2 = 4.50, P <0.05; 22.5% vs 5%; X ~ 2 = 5.16, P <0.05). Conclusion HS1 may be a new tumor-testis antigen gene. It is necessary to further study the biological function and clinical value of these positive clones.