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【目的】研究转GO基因番茄的抗病机制。【方法】通过测定转GO基因番茄和对照未转基因番茄接种叶霉菌Fulviafulva后,体内超氧物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性变化。【结果】接种后转基因番茄株系的SOD、POD、CAT活性均比对照明显增加,且转基因番茄的活性高峰比对照早。转基因番茄在接种后PAL和PPO活性都出现2个活性高峰,且第2个峰值比第1个高,而对照只有1个活性高峰,其总体水平明显低于转基因番茄。用番茄叶霉病(Fulviafulva)病原菌1.2.3.4生理小种侵染T1代转基因植株。【结论】结果显示转基因番茄植株的抗病性有不同程度的提高,多数发病时间推迟,病情明显减轻。
【Objective】 The objective of this study was to study the mechanism of disease resistance in GO transgenic tomato. 【Method】 After inoculation of Fulvia fulva with GO gene transgenic tomato and control non-transgenic tomato leaves, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) activity changes. 【Result】 The activity of SOD, POD and CAT in transgenic tomato plants after inoculation were significantly increased compared with the control, and the peak activity of transgenic tomato was earlier than that of the control. The activity of PAL and PPO in transgenic tomato showed two activity peaks after inoculation, and the second peak was higher than the first one, while the control had only one activity peak, the overall level was obviously lower than that of the transgenic tomato. T1 generation transgenic plants were infected with 1.2.3.4 physiological races of Fulvia fulva pathogen. 【Conclusion】 The results showed that the resistance of transgenic tomato plants increased in varying degrees, most of the onset time was delayed, the disease significantly reduced.