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目的观察活血解毒方对糖尿病大鼠周围神经病变的疗效及作用机制。方法将50只雄性SD大鼠分为正常组、模型组、甲钴胺组、活血解毒方不同剂量组。除正常组外,其余各组予链脲佐菌素腹腔注射(65μg/g)造模,并给予相应的干预措施,16周后观察大鼠血糖、热板潜伏期、神经传导速度、坐骨神经病理的变化情况,检测坐骨神经中蛋白激酶C(PKC)的表达及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量及总抗氧化能力。结果与正常组比较,模型组大鼠血糖显著升高,热板潜伏期延长,神经传导速度减慢,髓鞘松解,血清中SOD活性降低、MDA升高、总抗氧化能力减弱,坐骨神经内PKC表达增加,差异有统计学意义(P<0.05,P<0.01)。与模型组比较,活血解毒方高、低剂量组热板潜伏期缩短,神经束膜整齐,PKC在坐骨神经内表达减少,血清中MDA降低,活血解毒方高剂量组神经传导速度增快,差异有统计学意义(P<0.05)。结论活血解毒方可改善糖尿病大鼠周围神经病变,其机制可能与抑制PKC及MDA有关。
Objective To observe the curative effect and mechanism of Huoxue Jiedu Fang on peripheral neuropathy in diabetic rats. Methods Fifty male SD rats were divided into normal group, model group, mecobalamin group and different dosage groups of Huoxue Jiedu Decoction. Except for the normal group, all the other groups were given intraperitoneal injection of streptozotocin (65μg / g), and the corresponding interventions were given. After 16 weeks, the blood glucose, the latency of the hot plate, the nerve conduction velocity, the sciatic nerve pathology The expression of protein kinase C (PKC) and the contents of superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidant capacity in the sciatic nerve were detected. Results Compared with the normal group, the blood glucose in the model group was significantly increased, the hot plate latency was prolonged, the nerve conduction velocity was slowed down, the myelin sheath was loosened, the serum SOD activity was reduced, the MDA was increased, the total antioxidant capacity was reduced, the sciatic nerve PKC The expression increased, the difference was statistically significant (P <0.05, P <0.01). Compared with the model group, the incubation period of Huoxue Jiedu Decoction shortened, the neuraxial membrane became neat, the expression of PKC in the sciatic nerve decreased, MDA in the serum decreased and the nerve conduction velocity increased in the high-dose Huoxue Jiedu Fang group Significance (P <0.05). Conclusions Huoxue Jiedu decoction can improve peripheral neuropathy in diabetic rats, which may be related to the inhibition of PKC and MDA.