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目的观察野菊花提取物对糖尿病肾病大鼠的影响及作用机制。方法将48只SD大鼠,♀,随机分为正常对照组、模型对照组和野菊花低、中、高剂量组和阳性药依帕司他组(n=8)。除正常对照组外,其余各组大鼠腹腔注射链脲佐菌素35 mg·kg-1,成功造模后野菊花组分别给予1,2,4 g·kg-1野菊花提取物,依帕司他组给予10 mg·kg-1依帕司他,连续灌胃8周后,检测大鼠血糖、体质量、肾脏脏体比、24 h尿白蛋白量、肾组织超氧化歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)的含量;HE染色检测肾脏病理改变,ELISA法检测醛糖还原酶(AR)活性并测定其基因表达。结果与正常对照组相比,模型对照组上述指标除CAT外均有显著改变;与模型对照组相比,野菊花能明显降低大鼠血糖水平、AR活性及其基因表达,明显减少24 h尿白蛋白,升高肾组织SOD、CAT活性,显著降低MDA含量。结论野菊花提取物对大鼠糖尿病肾病有一定治疗作用,在调节血糖的同时,可能通过提高机体抗氧化能力、减少肾脏AR活性与基因表达以抑制多元醇通路的激活发挥其肾功能的保护作用。
Objective To observe the effect of Chrysanthemum extract on diabetic nephropathy rats and its mechanism. Methods 48 SD rats were randomly divided into normal control group, model control group and low, medium and high dose wild chrysanthemum group and epalrestat group (n = 8). Rats in each group were given intraperitoneal injection of streptozotocin 35 mg · kg-1, except for the normal control group, and the wild chrysanthemum group was given 1,2,4 g · kg-1 wild chrysanthemum extract respectively, The patients in the paclitaxel group were given 10 mg · kg-1 epalrestat. After 8 weeks of continuous gavage, blood glucose, body weight, ratio of renal viscera, 24-hour urinary albumin, renal superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were measured. The pathological changes of kidney were examined by HE staining. The activity of aldose reductase (AR) was measured by enzyme linked immunosorbent assay (ELISA) Results Compared with the normal control group, the above indexes in the model control group were significantly changed except CAT. Compared with the model control group, the wild chrysanthemum could significantly reduce the blood glucose level, AR activity and gene expression in rats, Albumin, increased SOD and CAT activity in renal tissue, significantly reduced MDA content. Conclusion Wild Chrysanthemum extract has a therapeutic effect on diabetic nephropathy in rats, and it may play a protective role on renal function by regulating the antioxidant capacity, reducing renal AR activity and gene expression, and inhibiting the activation of polyol channels, while regulating blood glucose .