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目的建立同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄色素A含量的方法。方法采用Welchrom C18色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸(B)为流动相,梯度洗脱;流速:1.0 m L·min-1;检测波长:230 nm。结果芍药苷在7.02~52.64μg·m L-1内呈良好的线性关系(r=0.999 7),平均回收率为99.3%,RSD为1.2%;丹酚酸B在23.19~173.90μg·m L-1内呈良好的线性关系(r=0.999 7),平均回收率为98.2%,RSD为1.1%;羟基红花黄色素A在4.47~33.50μg·m L-1内呈良好的线性关系(r=0.999 3),平均回收率为98.0%,RSD为1.0%。结论本法简便、灵敏、准确、重复性好,可用于冠心康颗粒的质量控制。
Objective To establish a method for simultaneous determination of paeoniflorin, salvianolic acid B and hydroxysafflor yellow A in Guanxinkang granules. Methods The mobile phase was eluted with a gradient of acetonitrile (A) - 0.1% phosphoric acid (B) on a Welchrom C18 column (250 mm × 4.6 mm, 5 μm) with a flow rate of 1.0 mL · min-1 and a detection wavelength of 230 nm. Results Paeoniflorin had a good linearity (r = 0.999 7) in the range of 7.02-52.64μg · m L-1 with an average recovery of 99.3% and a RSD of 1.2%. Salvianolic acid B was in the range of 23.19-173.90μg · m L (R = 0.999 7), the average recovery was 98.2% and the RSD was 1.1%. There was a good linear relationship between hydroxysafflor yellow A and 4.47 ~ 33.50 μg · m L -1 r = 0.999 3) with an average recovery of 98.0% and a RSD of 1.0%. Conclusion This method is simple, sensitive, accurate and reproducible. It can be used for the quality control of Guanxinkang granules.