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蔓生性(vining growth habit,VGH)是野生大豆茎的生长习性,对其遗传规律的研究有助于全面了解大豆株型及驯化特点。以南农86-4×PI342618B种间杂交衍生的重组自交系群体NJRINP的亲本和286个家系为材料,利用含226个标记的遗传图谱,采用Win QTLCart 2.5软件的复合区间作图法对2013和2014年开花期(R1)和成熟期(R8)蔓生性数据进行了QTL定位分析。结果开花期检测到4个蔓生性QTL,分别位于D1a(Chr.1)、G(Chr.18)和L(Chr.19)连锁群,其中q VGH-D1a、q VGH-G-1和q VGH-G-2两年均能检测到;q VGH-G-2两年的贡献率分别达14.16%和14.18%,是控制开花期蔓生性的稳定主效QTL。成熟期蔓生性两年均检测到位于G和L连锁群上的q VGH-G-1和q VGH-L位点,其中q VGH-G-1在开花期和成熟期的表型贡献率相当,是稳定表现的QTL;而q VGH-L由于贡献率大(两年R2分别为39.11%和23.14%),是控制成熟期蔓生性的主效QTL,其可能与结荚习性基因相关。促进蔓生性的等位基因均来自野生大豆PI342618B,但控制开花期和成熟期蔓生性的遗传体系不尽相同。
Vigor growth habit (VGH) is the growth habit of wild soybean stems. The study of its genetic law will help to understand the plant type and domestication characteristics of soybean. Nongnong 86-4 × PI342618B interspecific hybridization of recombinant inbred line population NJRINP parents and 286 pedigrees as the material, using 226 markers of genetic map, the use of Win QTLCart 2.5 composite interval mapping of 2013 And QTLs for flowering (R1) and maturity (R8) in 2014 were analyzed. Results Four clonal QTLs were detected at the flowering stage, which were located in the linkage group D1a (Chr.1), G (Chr.18) and L (Chr.19), respectively. The QVGH-D1a, qVGH-G- VGH-G-2 could be detected in both years. The contribution rate of VGH-G-2 in two years was 14.16% and 14.18%, respectively. It was the main QTL for controlling vines growth in flowering stage. The loci of q VGH-G-1 and q VGH-L located on the G and L linkage groups were detected in the two years of creeping development in mature stage. The phenotypic contribution of q VGH-G-1 at flowering and maturity was comparable , Which was a stable QTL for QTLs. However, qVGH-L was the major QTL for controlling the invasiveness of mature rice due to its high contribution rate (39.11% and 23.14% respectively over two years), which may be related to the pod locus genes. The alleles that promote invasiveness are derived from wild soybean PI342618B, but the genetic systems that control the invasiveness at flowering and maturity vary.