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Summary: To set up a method of amplification for the whole CagA gene of Helicobacter pylori andits fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employedin combination with TD-PCR to amplify the gene and EcoRI and Hind Ⅲ were used to generate theRFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplifiedand the relevant RFLP fingerprintings were obtained. It is concluded that the method can be usedto amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.