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该项研究目的是通过对肾移植大鼠的饮食营养干预,观察大豆异黄酮对慢性移植肾肾病的防治作用。方法采用选择近交系雄性Fisher (F344)大鼠作为供者,雄性Lewis(Lew)大鼠作为受者,采用显微外科技术制作肾移植模型。将受者随机分为三组,分别给予高异黄酮大豆蛋白饲料(HIS组)、低异黄酮大豆蛋白饲料(LIS组)或酪蛋白饲料(CAS组)。移植前和移植后第4、12和24周时检测血压,并收集受者的血和尿样,检测尿蛋白和血肌酐含量。24周时处死大鼠获取移植肾,行组织学和免疫组织化学检查。实验结果,在移植后4周时,HIS组受者的尾动脉收缩压、24h尿蛋白含量和血肌酐浓度即低于LIS组和CAS组,但差异无统计学意义(P>0.05);移植后12周和24周时,HIS组的受者尾动脉收缩压、24h尿蛋白含量和血肌酐浓度均较LIS组和CAS组显著降低(P<0.05);移植后24周时,HIS组移植肾组织的间质纤维化和炎症、血管硬化、肾小球硬化和肾小管萎缩等慢性病变均较LIS组和CAS组为轻(P<0.05);HIS组移植肾组织中转化生长因子-β1 (TGF-β1)的表达和分泌均较LIS组和CAS组为少(P<0.05)。由此得出结论,大豆异黄酮对移植肾功能和结构有保护作用,可作为一种防治慢性移植肾肾病的新方法。
The purpose of this study was to observe the preventive and therapeutic effects of soybean isoflavones on chronic allograft nephropathy by dietary nutrition intervention in kidney transplanted rats. Methods The inbred male Fisher (F344) rats were selected as donors, and male Lewis (Lew) rats were used as recipients. Microsurgical techniques were used to make kidney transplantation models. The recipients were randomly divided into three groups and were given high isoflavone soy protein feed (HIS group), low isoflavone soy protein feed (LIS group) or casein feed (CAS group). Blood pressure was measured before transplantation and at 4, 12 and 24 weeks after transplantation, blood and urine samples were collected from the recipients, and urinary protein and serum creatinine levels were measured. The rats were sacrificed at 24 weeks to obtain transplanted kidneys for histological and immunohistochemical examinations. As a result of the experiment, at the 4th week after transplantation, the tail artery systolic blood pressure, 24-hour urinary protein content, and serum creatinine of the HIS group were lower than those of the LIS group and the CAS group, but the difference was not statistically significant (P>0.05); After 12 and 24 weeks, the tail artery systolic blood pressure, 24-hour urinary protein and serum creatinine levels in the HIS group were significantly lower than those in the LIS group and the CAS group (P<0.05); 24 weeks after transplantation, the HIS group was transplanted Renal interstitial fibrosis and inflammation, arteriosclerosis, glomerulosclerosis and renal tubular atrophy were all milder than those in LIS and CAS groups (P<0.05). Transforming growth factor-β1 in renal tissue of HIS group The expression and secretion of TGF-β1 were lower than those in the LIS and CAS groups (P<0.05). It was concluded that soy isoflavones have a protective effect on the function and structure of transplanted kidneys and can be used as a new method for the prevention and treatment of chronic allograft nephropathy.