细胞成像技术是研究细胞运动、分裂和凋亡的必要手段,所以荧光条件下的细胞成像技术显得尤为重要。为满足荧光细胞观察对微弱光信号的检测和成像的要求,文章通过结构和电路设计,研制了一种用于荧光细胞观察的制冷CCD成像系统。通过分析各组件的技术特点,设计了高效制冷结构,提高了细胞荧光观察输出图像质量。 Cell imaging is a necessary tool to study cell motility, cell division and apoptosis. Therefore, cell imaging under fluorescence conditions is particularly important. In order to meet the requirements of detecting and imaging weak light signals observed by fluorescent cells, a refrigeration CCD imaging system for fluorescent cell observation was developed through structural and circuit design. By analyzing the technical characteristics of each component, an efficient refrigeration structure is designed to improve the output image quality of cell fluorescence observation.