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Objective:To labelavidin(Av)or streptavidin(SA)with 153 Sm by takingadvantageof thehighbindingaffin-ityof biotinto Av or SA.Methods:A biotinderivative(DTPA-biotin)wasradiolabelledwith 153 Sm andthenboundto Av or SA.Thein vivo kineticsandbiodistributionof 153 Sm-labeledAv,SA andDTPA-biotinwerestudiedinratsandmice.Results:153 Sm-Avwascharacterizedby rapidclearancefromthebloodwithhighliverandrenaluptake;153 Sm-SAwas clearedfromthebloodslowlywithhighretentionintheliver,spleenandkidney,whereas 153 Sm-DTPA-biotinmetabolismwas accelerated,anditsexcretionwasmainlythroughthekidney.Conclu sion:Thebiodistributiondifferenceof SAandAvmay providean experimentalbasisfor theselectionof differentcomponentsof avidin-biotinsystemin pretagetingradioim-munoimagingandradioimmunotherapy.
Objective: To labelavidin (Av) or streptavidin (SA) with 153 Sm by taking advantage of the high binding specificity-biotinto Av or SA. Methods: A biotinderivative (DTPA-biotin) was radiolabelled with 153 Sm and inboundto Av or SA.The in vivo kineticsandbiodistributionof 153 Sm-labeledAv, SA andDTPA-biotinwerestudiedinratsandmice.Results: 153 Sm-Avwascharacterizedby rapidclearancefromthebloodwithhighliverandrenaluptake; 153 Sm-SAwas clearedfromthebloodslowlywithhighretentionintheliver, spleenandkidney, whereas 153 Sm-DTPA-biotinmetabolismwas accelerated, anditsexcretionwasmainlythroughthekidney.Conclu sion: Thebiodistributiondifferenceof SAandAvmay providean experimentalbasisfor theselectionof differentcomponentsof avidin-biotinsystemin pretagetingradioim-munoimagingandradioimmunotherapy.