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目的：观察伯氏疟原虫（Ｐ．ｂｅｒｇｈｅｉ）小亚单位核糖体ＲＮＡ（ＳＳＵｒＲＮＡ）在斯氏按蚊体内的转录类型和动态。方法：应用ＲＴ－ＰＣＲ方法，特异地扩增伯氏疟原虫Ａ型和Ｓ型ＳＳＵｒＲＮＡ，检测斯氏按蚊虫体内Ｓ型ＳＳＵｒＲＮＡ的转录水平。结果：吸感染血的斯氏按蚊体内仅见Ｓ型ＳＳＵｒＲＮＡ的条带。整体检测从第２天开始查见，第８天明显增强，第１２～１６天达到高峰。在蚊腹部Ｓ型ＳＳＵｒＲＮＡ转录动态与整体检测结果相似。胸部于第１２天查见该基因的转录。结论：斯氏按蚊体内伯氏疟原虫仅转录Ｓ型ＳＳＵｒＲＮＡ，它的转录动态与疟原虫在蚊体内的发育密切相关。
Objective: To observe the transcriptional types and dynamics of P. berghei small subunit ribosomal RNA (SSUrRNA) in An. Methods: RT-PCR was used to amplify the type A and type S SSR rRNA of Plasmodium berghei to detect the transcription level of S-type SSUrRNA in the mosquitoes. Results: Only S-type SSUrRNA bands were found in the infected Anopheles stephensi. The overall test from the first two days of inspection, significantly enhanced on the 8th, the first 12 to 16 days to reach the peak. The transcriptional activity of S-type SSUrRNA in mosquito abdomen was similar to the overall test. The thorax was found on the 12th day of the gene transcription. Conclusion: Plasmodium berghei in S. anopheles only transcribe S-type SSUrRNA, and its transcriptional activity is closely related to the development of Plasmodium in the mosquito.