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目的 探讨清开灵注射液对谷氨酸(Glu)神经毒性脑水肿时突触体内游离钙(［Ca2+］i)的影响。方法 30只大鼠分成正常对照组、模型组、治疗组3组，右侧脑室注射Glu制作大鼠神经毒性脑水肿模型，4 h后处死大鼠测定其脑皮质含水量、钠、钾、钙和突触体［Ca2+］i含量。结果 模型组脑含水量(82.04±0.8)%，钠含量(244.4±29.3) mmol/kg，［Ca2+］i (327.9±33.2) nmol/L分别较正常对照组(79.93±0.59)%，(213.8±13.0) mmol/kg，(159.6±18.6) nmol/L显著升高(P<0.01，P<0.05，P<0.01)，模型组钙(4.76±0.74) mmol/kg较正常对照组(5.21±0.81) mmol/kg显著降低(P<0.05)。治疗组脑含水量(80.13±0.72)%，钠含量(209.4±14.7) mmol/kg，［Ca2+］i (162.5±17.6) nmol/L较模型组显著降低(P均<0.01)，钙(5.44±0.76 mmol/kg.干重)却增高(P<0.05)。结论 清开灵对大鼠谷氨酸神经毒性脑水肿有保护作用，其机制可能与拮抗突触体钙离子内流有关。
Objective To investigate the effect of Qingkailing injection on synaptosomal free calcium ([Ca2+]i) during glutamate (Glu) neurotoxic brain edema. Methods Thirty rats were divided into normal control group, model group, and treatment group. The rats were injected with Glu in the right ventricle to produce a model of neurotoxic brain edema. The rats were sacrificed 4 hours later to determine their cerebral cortical water content, sodium, potassium and calcium. And synaptosome [Ca2+]i content. Results The brain water content (82.04±0.8)%, sodium content (244.4±29.3) mmol/kg, and [Ca2+]i (327.9±33.2) nmol/L in model group were higher than those in normal control group (79.93±0.59)%,(213.8). (13.0) mmol/kg, (159.6±18.6) nmol/L was significantly increased (P<0.01, P<0.05, P<0.01), and calcium in the model group was (4.76±0.74) mmol/kg compared with the normal control group (5.21±) 0.81) mmol/kg decreased significantly (P<0.05). The brain water content (80.13±0.72)%, sodium content (209.4±14.7) mmol/kg, [Ca2+]i (162.5±17.6) nmol/L in the treatment group were significantly lower than those in the model group (P<0.01), calcium (5.44) ± 0.76 mmol/kg dry weight but increased (P < 0.05). Conclusion Qingkailing has a protective effect on brain edema induced by glutamate neurotoxicity in rats, and its mechanism may be related to its antagonistic effect on intracellular Ca2+ influx.