Cytological test and human skin test were used to study the antioxidant, anti-aging and moisturizing effects of a compound plant extract, and to evaluate its safety. The cytological test results showed that the compound extract could significantly scavenge the reactive oxygen species (ROS) generated by H2O2 stimulation and protect cells from oxidative damage. It can significantly inhibit the expression of matrix metalloproteinase -1 (MMP-1). The results of human skin test showed that the moisture content of cheek stratum corneum, R2 value and Q1 value of skin elasticity were significantly improved after 28 days of continuous use of the Makeup Base product added with the compound plant extract. The Ra value, Rz value and Rt value of skin texture around eyes have been significantly improved, which shows that the product has the effects of improving the moisture content of stratum corneum, improving skin elasticity and diminishing the fine lines around the eyes; the product has good safety.