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目的:建立用HPLC法测定复方玄驹胶囊中淫羊藿苷含量的方法。方法:采用Agilent Zoxbax·XDB-C_(18)柱(150mm×4.6mm,5μm);流动相:乙腈:水:磷酸=25:75:0.5;流速:1.0ml·min~(-1);检测波长:270nm。结果:淫羊藿苷进样量在0.07～1.12μg范围内具有良好的线形关系(r=0.999 7),加样回收率为99.27%,RSD为0.81%。结论:本方法灵敏、简便、快速,适合于该药的质量控制。
Objective: To establish a HPLC method for determination of icariin in Compound Xuanju Capsule. Methods: Agilent Zoxbax XDB-C 18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile: water and phosphoric acid at 25:75: 0.5. The flow rate was 1.0 ml · min -1. Wavelength: 270nm. Results: The calibration curve of icariin was linear in the range of 0.07-1.12 μg (r = 0.999 7). The recoveries of icariin was 99.27% and the RSD was 0.81%. Conclusion: The method is sensitive, simple and rapid, suitable for the quality control of the drug.