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材料与方法: 将20只健康牙纵行切开,在根尖区钻一孔,然后分成3组,第一组为6只对照牙,第二组7只牙培养于含蔗糖和变形链球菌的基础培养基中,第三组7只牙先以氟化亚锡处理,与第二组同样培养。使用保存于Todd-Hewitt培养基中的变形链球菌6175~a。基础培养基由胰酶水解物(Trypticase)2%、NaCl 0.2%、MnSO_40.0015%、MgSO_4 0.012%、K_2HPO_4 0.2%、K_2Co_3 0.1%、及蒸馏水1000毫升配成。再制备经过消毒的10%蔗糖液。氟化亚锡为新鲜
MATERIALS AND METHODS: Twenty healthy teeth were longitudinally dissected, a hole was drilled in the apical region, and then divided into three groups, the first group of six control teeth, the second group of seven teeth cultured in sucrose and Streptococcus mutans Of the basal medium, the third group of seven teeth first treated with stannous fluoride, and the second group of the same culture. Streptococcus mutans 6175-a stored in Todd-Hewitt medium were used. The basal medium consists of Trypticase 2%, NaCl 0.2%, MnSO 40.0015%, MgSO 4 0.012%, K 2 HPO 4 0.2%, K 2 Co 3 0.1%, and distilled water 1000 ml. Prepare sterilized 10% sucrose solution. Stannous fluoride is fresh