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目的 探讨性成熟期大鼠实验性精索静脉曲张 (EVC)附睾显微和超微结构的改变及其在不育中的作用。 方法 部分结扎左肾静脉建立大鼠EVC模型 ,常规制作附睾各段光镜及电镜标本。 结果 EVC大鼠附睾各段均出现程度不同且有区段特异性的损害。光镜下主要表现有间质血管充血扩张 ,淋巴细胞浸润、积聚 ,间质内出现精子肉芽肿。附睾管萎缩 ,上皮细胞变性 ,甚至出现空泡化 ,上皮内晕、亮细胞数明显增多 ;管腔内出现大量未成熟生精细胞、畸形精子等。电镜下主要表现为主细胞内溶酶体增多、变大 ,残余小体增加 ,细胞器 (内质网、线粒体、高尔基复合体等 )受损 ,部分主细胞内出现大空泡。晕细胞、亮细胞内溶酶体也增大、增多 ,亮细胞常膨大 ,游离面突入管腔。上皮细胞游离面微绒毛稀少 ,可见局灶性断裂和破坏。上皮基膜增厚。 结论 性成熟期大鼠EVC可致附睾管上皮显微及超微结构明显改变 ,这可能是精索静脉曲张相关不育的一个主要原因
Objective To investigate the changes of ultrastructure and ultrastructure of epididymis in experimental varicocele (EVC) in sexual maturity rats and its role in infertility. Methods Part of the left renal vein was ligated to establish rat model of EVC, routine epididymal segments of light and electron microscopy specimens. Results The epididymal segments of EVC rats all showed different degrees and had segment-specific damage. Light microscopy mainly showed interstitial vascular congestion and expansion, lymphocyte infiltration, accumulation, interstitial sperm granuloma. Epididymal tube atrophy, epithelial cell degeneration, and even vacuolization, intraepithelial halo, bright cells increased significantly; a large number of immature spermatogenesis within the lumen, abnormal sperm and so on. Electron microscopy mainly showed the increase of lysosomes in host cells, the increase of residual bodies, the damage of organelles (endoplasmic reticulum, mitochondria, Golgi complex, etc) and the appearance of large vacuoles in some major cells. Halo cells, bright intracellular lysosomes also increased, increased, bright bulbs often swollen, free surface into the lumen. Epithelial cells free surface microvilli scar, showing focal rupture and destruction. Epithelial basement membrane thickening. CONCLUSIONS: EVC in rats with mature stage can significantly change the microstructure and ultrastructure of epididymal epithelium, which may be one of the main causes of varicocele-related infertility