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目的观察眼镜蛇毒组分Ｃ对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２的抑瘤作用及其体外对肝癌Ｈ２２细胞存活率的直接影响。方法通过半体内实验，观察眼镜蛇毒组分Ｃ对小鼠腹水型肝癌Ｈ２２的抑瘤率；通过细胞培养，以细胞存活率为指标观察中华眼镜蛇毒组分Ｃ对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２细胞在体外的直接杀伤作用。结果眼镜蛇毒组分Ｃ能明显抑制ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２细胞的生长，其抑瘤作用随剂量增大而增强，ＩＣ５０为９５ｍｇ／Ｌ。在体外，眼镜蛇毒组分Ｃ的浓度对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２细胞存活率的影响随其作用剂量的增大而增强，ＩＣ５０为９ｍｇ／Ｌ；同时，这种影响还随其作用时间的延长而增强。结论眼镜蛇毒组分Ｃ对ＢＡＬＢ／Ｃ型小鼠腹水型肝癌Ｈ２２有显著的抑瘤作用。
Objective To observe the inhibitory effect of cobra venom component C on BALB/c mouse ascitic hepatocellular carcinoma H22 and its direct effect on the survival rate of hepatoma H22 cells in vitro. Methods Inhibitory rate of mouse cobra venom component C against mouse ascitic hepatocellular carcinoma H22 was observed by half-in vivo experiments. The cell viability was used as an index to observe the components of Chinese cobra venom component C for BALB/c mouse ascites. Direct killing effect of hepatoma H22 cells in vitro. Results Cobra venom component C could significantly inhibit the growth of BALB/c mouse ascitic hepatocellular carcinoma H22 cells. The antitumor effect of cobra venom component C increased with increasing dose. The IC50 was 95 mg/L. In vitro, the effect of concentration of cobra venom component C on the survival rate of BALB/c mouse ascitic hepatoma H22 cells increased with increasing dose, IC50 was 9mg/L; at the same time, this effect also followed The duration of the action increases. Conclusion Cobra venom component C has significant anti-tumor effect on BALB/c mouse ascitic hepatoma H22.