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本实验研究日本灵芝对小鼠脾细胞产生白细胞介素-2(IL-2)的影响。结果表明在试管内氢化考的松0.025～1μg/ml及环胞霉素A 0.05μg/ml可明显抑制小鼠脾细胞产生IL-2,P<0.01。灵芝在试管内能明显对抗氢化考的松及环胞霉素A的抑制作用而促进IL-2产生增加,其作用与药物浓度有关,10μg/ml以上有显著作用,P<0.01。灵芝不同浓度与氢化考的松同时对脾细胞进行预处理8 h后,灵芝可以明显减轻氢化考的松的抑制作用。整体研究亦证明灵芝可以促进脾细胞产生IL-2,即使在同时用免疫抑制剂的条件下,IL-2的产生仍有显著增加。
This experiment investigated the effects of Ganoderma lucidum on the production of interleukin-2 (IL-2) in mouse spleen cells. The results showed that in vitro hydrocortisone 0.025 to 1 μg/ml and cyclosecillin A 0.05 μg/ml could significantly inhibit IL-2 production in mouse spleen cells, P<0.01. Ganoderma lucidum can obviously inhibit the inhibition of hydrocortisone and cyclosporin A in vitro and promote the increase of IL-2 production, and its effect is related to the drug concentration. There is a significant effect at 10 μg/ml or more, P<0.01. Different concentrations of Ganoderma lucidum and hydrocortisone simultaneously pretreated splenocytes for 8 h, Ganoderma lucidum can significantly reduce the inhibitory effect of hydrocortisone. The overall study also showed that Ganoderma lucidum can promote the production of IL-2 by spleen cells. Even under the condition of simultaneous use of immunosuppressive agents, the production of IL-2 is still significantly increased.