论文部分内容阅读
目的:研究人巨细胞病毒不同临床分离株UL141基因多态性,并分析这种多态性与人巨细胞病毒先天感染疾病之间的关系。方法:对荧光定量PCR方法检测HCMV-DNA阳性的临床低传代分离株进行UL141基因全序列PCR扩增,对扩增阳性的标本进行测序及分析。结果:19株临床低传代分离株在Toledo株UL141基因227位均缺失一个碱基T,因此产生两个新的ORF(UL141A和UL141B)。与Toledo株相应片段比较,UL141A预测蛋白质第75位氨基酸后序列和翻译后修饰位点产生大量变异。UL141B高度保守。结论:临床分离株的UL141片段产生两个新的ORF。
Objective: To study the polymorphism of UL141 gene of different human cytomegalovirus isolates and to analyze the relationship between this polymorphism and human cytomegalovirus congenital infectious diseases. Methods: The full-length UL141 gene of HCMV-DNA positive low passage isolates were detected by real-time fluorescence quantitative PCR. The positive samples were sequenced and analyzed. Results: The 19 clinical isolates showed one base T deletion at 227 of UL141 gene in Toledo strain, resulting in two new ORFs (UL141A and UL141B). Compared with the corresponding fragment of Toledo strain, UL141A predicted a lot of variation in amino acid sequence and posttranslational modification site at amino acid 75 of the protein. UL141B highly conservative. Conclusion: Two novel ORFs were generated from the UL141 fragment of clinical isolates.