论文部分内容阅读
植物多功能特效营养素又叫SPNE ,SPNE有多种生化成份 ,对植物有营养、激活及促进植物生长发育的多种功能。它已广泛用于农业、林业、园林生产 ,大幅度提高蔬菜、果树、粮食、棉花、茶叶等多种作物产量和显著改善品质 ,它能促进植物生根、苗木快速生长。它能提高植物组织培养的出愈率、芽分化率、绿苗率、生根率。以不同作物 ,不同品种为材料 ,用SPNE进行不同类型、不同方式的处理。结果表明 ,仅用SPNE3浸种能提高发芽率和产量 ,仅用SPNE2喷施也能提高产量 ,而用SPNE3浸种后播种 ,再用SPNE2喷施植株 ,增产辐度最高。使用SPNE后所引起的各种表型的改变涉及到植物内在的生理生化过程的变化 ,其原因又在于与植物生理相关基因的表达调控的改变。为了研究SPNE诱导的基因表达的差异 ,我们采用差异显示技术 (differ entialdisplay) ,从基因转录水平探测经SPNE处理的辣椒幼苗基因产物的差别 ,实验证实SPNE确实可诱导基因的差别表达 ,使某些基因活化表达。将水稻常规品种用SPNE处理后的种子和对照的种子在同样条件下进行发芽 ,生长试验 ,结果表明 ,用SPNE处理后的优良性状能通过种子传递给下一代 ,即经SPNE处理后活化的基因在下一代中不再沉默 ,仍能表达。
Plant multifunctional special effects nutrients also known as SPNE, SPNE has a variety of biochemical ingredients, the plant nutrition, activation and promote plant growth and development of a variety of functions. It has been widely used in agriculture, forestry and landscape production and greatly improved the yield and quality of various crops such as vegetables, fruit trees, grain, cotton and tea. It can promote the rooting of plants and the rapid growth of seedlings. It can improve the plant tissue culture out of the rate of bud differentiation rate, green shoot rate, rooting rate. Different crops, different varieties of materials, using SPNE for different types and different ways of handling. The results showed that germination rate and yield could be increased by using SPNE3 soaking only. Spne3 spraying could also increase the yield. Soaking seed with SPNE3 and spraying SPNE2 with the highest yield. The changes of various phenotypes caused by the use of SPNE involve the changes of physiological and biochemical processes inherent in plants, which in turn is due to the changes of the expression regulation of genes related to plant physiology. In order to investigate the differences in gene expression induced by SPNE, we used differential display (differential entialdisplay) to detect the differences in SPNE-treated gene products of pepper seedlings from gene transcription level. Experiments confirmed that SPNE did induce differential expression of some genes, Gene activation expression. The germination and growth of the conventional rice seeds treated with SPNE and the control seeds were carried out under the same conditions. The results showed that the excellent traits after SPNE treatment can be transmitted to the next generation through SPNE, that is, the gene activated after SPNE treatment In the next generation no longer silent, still can express.