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小檗碱(Berberine)属季铵型异喹啉类生物碱。系黄连、黄柏、三颗针等中草药的主要有效成分,具有抗菌消炎作用。近年来发现小檗碱具有抗心律失常和正性肌力等心血管药理作用。生物样品中小檗碱的测定方法有纸色谱法,纸色谱-分光光度法,同位素标记法,气相色谱-质谱法。DL-樟脑-10-磺酸(CSA)是一种手性离子对试剂,曾用于生物胺的测定取得了良好的结果。我们用CSA作离子对试剂,建立了血浆中小檗碱的反相离子对色谱测定方法,并对GSA的色谱行为及方法的优化作了探讨。
Berberine is a quaternary ammonium isoquinoline alkaloid. Department of Coptis, Phellodendron, three needles and other main active ingredients of Chinese herbal medicine, with anti-bacterial anti-inflammatory effect. In recent years, berberine was found to have anti-arrhythmic and inotropic effects such as cardiovascular pharmacology. Determination of berberine biological samples include paper chromatography, paper chromatography - spectrophotometry, isotope labeling, gas chromatography - mass spectrometry. DL-camphor-10-sulfonic acid (CSA) is a chiral ion-pairing reagent that has been used to determine biogenic amines with good results. We used CSA as an ion pair reagent to establish a method for the determination of berberine in plasma by reversed-phase ion-pair chromatography. The chromatographic behavior of GSA and the optimization of the method were also discussed.