作者采用经过不同时间治疗的瘤型麻风病人皮肤活体组织的麻风杆菌接种于瑞士小白鼠足垫。在实验之前,排除了动物的自然分枝杆菌感染。其方法是将小鼠的足垫组织、大腿肌、肝、脾和少数其他脏器制成组织匀浆,涂片检查抗酸杆菌,并接种于改良罗氏培养基上,以除外任何可培养的分枝杆菌。作者认为,排除小鼠自然分枝杆菌感染是这项工作的重要前提,否则,实验结果可能由于这样的间发感染而受到损失。 The authors used leprosy bacilli from the skin of leprosy patients treated at different times to inoculate Swiss footpad. Prior to the experiment, animals were challenged with natural mycobacterial infection. The method is to make tissue homogenate of mouse foot pad tissue, thigh muscle, liver, spleen and a few other organs, smear acid-fast bacilli, and inoculate on modified Roche medium except for any culturable Mycobacterium. The authors believe that excluding mice with M. mycobacterial infection is an important prerequisite for this work, otherwise the experimental results may be lost due to such an episode of infection.