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目的:考察延胡索合剂对吡柔比星外渗性损伤的防治作用及机制。方法:建立小鼠吡柔比星外渗性损伤动物模型;观察模型对照组、延胡索合剂1组(外渗后30 min湿敷)、延胡索合剂2组(外渗后d 3湿敷)和如意金黄散组(外渗后30 min湿敷)的外渗性损伤面积、程度和愈合时间;各组在外渗后d 1,4,7,11,18取1只小鼠,切除损伤组织,HE染色观察病理形态学改变,免疫组化法检测损伤部位皮肤及皮下组织中的血管内皮生长因子(VEGF)和表皮生长因子受体(EGFR)的表达情况。结果:延胡索合剂组和如意金黄散组的外渗性损伤面积及程度明显低于对照组(P<0.05)。与其余3组相比,延胡索合剂1组外渗性损伤面积明显缩小,愈合时间缩短(P<0.05)。镜下观察显示,延胡索合剂1组损伤程度轻,溃疡发生较晚,组织修复早,损伤组织中VEGF和EGFR的表达明显上调,高峰提前。结论:延胡索合剂对小鼠吡柔比星外渗性损伤有良好的防治作用,疗效优于如意金黄散,其促进损伤愈合的机制可能与促使早期损伤组织释放EGFR和VEGF,促进纤维组织增生有关。
Objective: To investigate the preventive and therapeutic effects and mechanism of corydalis mixture on pirarubicin extravasation injury. Methods: The mouse model of pirarubicin extravasation injury was established. The model control group, corydalis group 1 (wet compress 30 min after extravasation), corydalis mixture 2 (wet dp 3) The area, degree and healing time of extravasation injury of Jinhuang powder group (30 min post-extravasation) were taken out. At 1, 4, 7, Pathological changes were observed by staining. The expression of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in the skin and subcutaneous tissue were detected by immunohistochemistry. Results: The area and degree of extravasation injury of Corydalox group and Ruyi Jinshuang San were significantly lower than those of the control group (P <0.05). Comparing with the other three groups, the area of extravasation lesion of the Corydalis Mixture Group 1 was significantly reduced and the healing time was shortened (P <0.05). Microscopic observation showed that there was a slight degree of lesion in the Cushing group and a late onset of ulcer. The expression of VEGF and EGFR in the early and injured tissues were significantly up-regulated and peaked earlier. CONCLUSION: Corydalis mixture has a good preventive and therapeutic effect on the pirarubicin extravasation injury in mice, and its curative effect is better than that of Ruyi Jinshuang San. The mechanism of its promoting wound healing may be related to promoting the release of EGFR and VEGF and promoting the proliferation of fibrous tissue in early injury tissues .