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为有效利用抗褐飞虱水稻Swarnalata,对2013年南京种植的Swarnalata/02428 F2分离群体进行抽穗期和种子休眠性考察,利用172个分子标记构建了Swarnalata/02428 F2的分子遗传连锁图谱,图谱全长为3311.4c M,标记间平均图距为19.22c M。利用Windows QTL Cartographer V2.5软件对该分离群体进行抽穗期和种子休眠性相关QTL检测,共检测到7个抽穗期相关QTL,分别位于第2、3、6、11染色体,其中位于第11染色体的q HD-11-1贡献率最高,为28.85%;检测到3个种子休眠性相关QTL,分别位于第3、6、9染色体,其中位于第9染色体的q Sd-9贡献率最高,为22.11%。分析表明,本研究检测到的抽穗期QTL与种子休眠QTL所在位置不同,说明该群体中种子休眠与抽穗期没有直接关系,它们分别由不同基因控制。本研究不仅为水稻休眠基因的精细定位及克隆奠定基础,也为更有效利用Swarnalata中的抗褐飞虱基因提供基础和一些优良的中间材料。
In order to effectively utilize the rice plant Swarnalata which resistant to BPH, the heading date and seed dormancy of Swarnalata / 02428 F2 population planted in Nanjing in 2013 were investigated. The molecular genetic linkage map of Swarnalata / 02428 F2 was constructed with 172 molecular markers, 3311.4c M, the average distance between markers was 19.22cM. QTLs related to heading and seed dormancy were detected by using Windows QTL Cartographer V2.5 software. Seven QTLs for heading date were detected in chromosomes 2, 3, 6 and 11 respectively, of which QTL was located on chromosome 11 Q HD-11-1 was the highest, accounting for 28.85%. Three QTLs related to dormancy were detected on chromosomes 3, 6 and 9, respectively, of which q Sd-9 was the highest on chromosome 9 22.11%. The results showed that QTLs for heading date and seed dormancy were different in this study, indicating that there is no direct relationship between seed dormancy and heading date in this population and they are controlled by different genes respectively. This study not only laid a foundation for the fine mapping and cloning of dormancy genes in rice, but also provided the basis for more effective utilization of the BPH genes in Swarnalata and some excellent intermediate materials.