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目的了解安徽省中北部地区淡色库蚊对多种杀虫剂的抗药性现状及其kdr基因突变情况。方法 2014年6-9月在淮北市、蚌埠市和滁州市3地采集淡色库蚊幼虫,带回实验室饲养至成虫,采用WHO成蚊接触筒药膜滤纸接触法对其进行0.05%溴氰菊酯、5%马拉硫磷、0.1%噁虫威、4%DDT抗性生物测定。以PCR扩增经溴氰菊酯抗性测定的库蚊抗性相关kdr基因并进行测序,统计L1014位点突变情况。结果上述3个地区淡色库蚊对溴氰菊酯、马拉硫磷、噁虫威、DDT 4种杀虫剂都产生了不同程度的抗药性,对DDT产生的抗性较高;虽然3地淡色库蚊接触DDT后的死亡率差异无统计学意义(F=1.027,P>0.05);但接触溴氰菊酯、马拉硫磷、噁虫威后的死亡率差异均有统计学意义(F=23.823、33.955、128.841,P均<0.01)。3地淡色库蚊种群的kdr基因1014位点均存在L1014F、L1014S这2种非同义突变;L1014F突变频率与溴氰菊酯抗性水平呈正相关(r2=0.718,P<0.01)。结论安徽省中北部地区淡色库蚊对溴氰菊酯、马拉硫磷、噁虫威、DDT均产生了较强的抗性,kdr基因L1014F突变频率与溴氰菊酯抗性水平呈正相关;各地区卫生部门需加强对媒介蚊虫抗性的动态监测。
Objective To understand the resistance status of multi-insecticides to Culex pipiens pallens in central and northern part of Anhui Province and the mutation of kdr gene. Methods June-September 2014, Culex pipiens pallens larvae were collected from 3 sites in Huaibei, Bengbu and Chuzhou, and the animals were reared in the laboratory until adults were infected. Inulin, 5% Malathion, 0.1% Oxacillin, 4% DDT resistant bioassay. The kdr gene of Culex pipiens quinquefasciatus resistant to deltamethrin was amplified by PCR and sequenced. The mutation of L1014 was analyzed. Results Culex pipiens pallens developed resistance to deltamethrin, malathion, fenvaleramide and DDT in the three areas above, and had higher resistance to DDT. Although three Mortality of Culex pipiens pallens exposed to DDT was not significantly different (F = 1.027, P> 0.05). However, the mortality rates of deltamethrin, malathion and fenvalerate were all significantly different F = 23.823,33.955,128.841, P <0.01). There were two non-synonymous mutations L1014F and L1014S in the kdr gene 1014 locus in 3 populations of Culex pipiens pallens. The mutation frequency of L1014F was positively correlated with the resistance level of deltamethrin (r2 = 0.718, P <0.01). Conclusion Culex pipiens pallens in central and northern part of Anhui Province have strong resistance to deltamethrin, malathion, vicina and DDT. The frequency of L1014F mutation in kdr gene is positively correlated with the resistance to deltamethrin. Health authorities in all regions need to strengthen the dynamic monitoring of vector mosquito resistance.