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目的 探讨儿童异型淋巴细胞 (异淋 )增多的原因和免疫学特点。方法 对 75例外周血涂片异淋 >0 10的患儿 ,应用ELISA方法检测柯萨奇病毒 (CVB)抗原及IgM抗体、EB病毒 (EBV)IgM抗体、巨细胞病毒(CMV)IgM抗体、腺病毒 (AdV)IgM抗体、乙肝病毒表面抗原、肺炎支原体 (MP)IgM抗体及采用咽试子分泌物PCR法检测DNA ;同时用流式细胞仪检测外周血T细胞亚群。结果 74例 (96 6 % )被检出有病毒或支原体感染 ;CD3+T细胞和CD8+T细胞比例明显增高 ,CD4 +T细胞和CD4 +/CD8+细胞比例明显降低 ,与对照组相比差异有非常显著性意义 (P <0 0 1) ,而传染性单核细胞增多症与传染性单核细胞增多综合征之间差异无显著意义 (P >0 0 5 )。结论 EBV、CVB、CMV、AdV和MP感染均可引起外周血异淋升高 ,其中以EBV最为明显 ;一旦外周血中出现大量异淋 ,则提示机体内存在免疫功能紊乱。
Objective To investigate the causes and immunological features of childhood dysplasia (heterophylla). Methods Serum levels of CVB antigen, IgM antibody, Epstein-Barr virus (EBV) IgM antibody, cytomegalovirus (CMV) IgM antibody, AdV IgM antibody, hepatitis B virus surface antigen, Mycoplasma pneumoniae (MP) IgM antibody and PCR by phagocytosis of the pharynx were used to detect DNA. Meanwhile, T lymphocyte subsets in peripheral blood were detected by flow cytometry. Results 74 cases (96.6%) were infected with virus or mycoplasma. The proportion of CD3 + T cells and CD8 + T cells was significantly increased, and the proportion of CD4 + T cells and CD4 + / CD8 + cells was significantly lower than that of the control group (P <0.01). However, there was no significant difference between infectious mononucleosis and infectious mononucleosis syndrome (P> 0.05). Conclusion EBV, CVB, CMV, AdV and MP infection can cause peripheral blood leaching increased, especially in EBV most obvious; once a large number of different lymphocytes in the peripheral blood, suggesting the presence of immune dysfunction in the body.