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提取正常男性外周血中性粒细胞,将其分为空白组(A组)及淋球菌组(B组)。在0,3,8,12小时分别以荧光定量RT-PCR测定各组中性粒细胞中iNOSmRNA及淋球菌norRmRNA含量,用Griess法检测各组细胞培养液中的一氧化氮(NO)浓度。与A组对比,B组中iNOs、norBmRNA表达及水平均升高;NO浓度在8小时达到高峰,以后逐渐下降(P<0.05)。而A组中各时间段iNOS、norBmRNA的表达及NO浓度无明显变化。B组norB表达与NO成正相关(P<0.01),而在norB上调后NO浓度逐渐下降。淋球菌感染中性粒细胞引起的iNOS表达上升,并产生NO抵抗淋球菌,而淋球菌norBmRNA上调,可能与该菌抵抗NO攻击并在中性粒细胞中生存和致病中起重要作用。
Normal male peripheral blood neutrophils were extracted and divided into blank group (group A) and gonococcal group (group B). The levels of iNOS mRNA and gonorrhea norr mRNA in neutrophils of each group were measured by fluorescence quantitative RT-PCR at 0, 3, 8 and 12 hours respectively. The concentrations of nitric oxide (NO) in each group were detected by Griess method. Compared with group A, the expression and levels of iNOs and norB mRNA in group B were increased; the concentration of NO peaked at 8 hours and then decreased gradually (P <0.05). In group A, the expression of iNOS and norB mRNA and the concentration of NO did not change significantly in each time period. There was a positive correlation between norB expression and NO in group B (P <0.01), but not in NO group. Neisseria gonorrhoeae infection caused by neutrophils increased iNOS expression and produce NO against Neisseria gonorrhoeae, and Neisseria gonorrhoeae norB mRNA upregulation, the bacteria may resist NO attack and play an important role in neutrophil survival and pathogenesis.