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为了检测贝类标本中的甲型肝炎病毒,我们建立了敏感特异的免疫捕获—逆转录—聚合酶链反应技术(IC-RT-PCR)。应用该技术检测不同地区采集的贝类标本中的HAV-RNA后发现:46份贝类标本有29份为阳性,检出率达6304%(29/46),其中毛蚶为6316%(12/19),文蛤为9000%(9/10),蚶子为5455%(6/11),说明各种贝类有不同程度的污染;南部、中部地区贝类标本中的HAV-RNA检出率较高,北部地区10份标本仅检出1份阳性,这与当地甲肝发病率较低一致。
To detect Hepatitis A virus in shellfish specimens, we established a sensitive and specific immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR). Using this technique to detect HAV-RNA in shellfish samples collected from different areas, 29 samples of 46 shellfish samples were positive, with a detection rate of 6304% (29/46), of which 6316 (12/19), clam 9000% (9/10), clam 5455% (6/11), which showed that shellfish had different degrees of pollution. Shellfish specimens from southern and central China In the detection rate of HAV-RNA is higher, 10 samples in the northern region only detected a positive, which is consistent with the lower incidence of local hepatitis A.