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目的评价人甲型H7N9禽流感全病毒灭活疫苗在小鼠中的免疫原性。方法分别将H7N9与H1N1、H5N1全病毒灭活疫苗稀释至血凝素抗原(hemagglutinin,HA)浓度分别为30、6、1.2、0.24和0.048μg/ml后,免疫BALB/c小鼠,各10只,0.5 ml/只,采用血凝抑制(hemagglutinin inhibition,HI)试验检测免疫后小鼠血清抗体滴度,计算抗体几何平均滴度(GMT)及半数有效剂量(ED_(50)),并检测免疫后小鼠血清对其他亚型病毒的HI滴度。结果 H7N9组小鼠阳转率与H1N1组较相近,仅H7N9 0.6μg剂量组GMT明显低于H1N1同剂量组(P<0.001),其余剂量组差异无统计学意义(P>0.05);H7N9与H1N1、H5N1全病毒灭活疫苗免疫小鼠ED_(50)分别为0.27、0.12和15μg HA。免疫后血清对其他亚型病毒无交叉反应。结论 H7N9疫苗在小鼠中的免疫原性略低于H1N1疫苗,但明显优于H5N1疫苗。
Objective To evaluate the immunogenicity of a human H7N9 avian influenza virus inactivated vaccine in mice. Methods BALB / c mice were immunized with 10, 6, 1.2, 0.24 and 0.048 μg / ml of H7N9 and H1N1 and H5N1 virus inactivated vaccine respectively The serum antibody titers of mice were measured by hemagglutinin inhibition (HI) test and the geometric mean antibody titer (GMT) and half effective dose (ED 50) were calculated and tested HI serum titer of other subtype viruses after immunization. Results The positive rate of mice in H7N9 group was similar to that in H1N1 group. The GMT of H7N9 0.6μg group was significantly lower than that of H1N1 group (P <0.001), but there was no significant difference between the other groups (P> 0.05) The mice immunized with H1N1 and H5N1 whole virus inactivated vaccines had ED_ (50) of 0.27, 0.12 and 15 μg HA, respectively. Post-immunization sera did not cross-react with other subtypes of the virus. Conclusion The immunogenicity of H7N9 vaccine in mice was slightly lower than that of H1N1 vaccine, but significantly better than H5N1 vaccine.