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本试验旨在研究过瘤胃胆碱(RPC)对成年黔北麻羊瘤胃发酵及血浆生化指标的影响。以体况良好,年龄、体重[(35.0±1.2)kg]一致的成年黔北麻羊6只为试验动物,分为对照组和试验组,每组3个重复,每个重复1只羊。采用交叉试验设计,对照组饲喂基础饲粮,不含RPC;试验组补饲10 g/d的RPC。试验持续30 d,分2期,每期15 d;每期最后1 d采集瘤胃液测定p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸(乙酸、丙酸、丁酸)浓度、纤维素酶(微晶纤维素酶、羧甲基纤维素酶、纤维二糖酶、木聚糖酶)活性,同时采血测定血浆葡萄糖、尿素氮、总蛋白、白蛋白、总胆固醇、甘油三酯、肌酐浓度。结果显示:1)RPC对山羊采食速度没有影响,对采食量没有显著影响(P>0.05);2)RPC对瘤胃液p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸浓度及4种纤维素酶活性影响较小,2组间差异均不显著(P>0.05);3)RPC对血浆生化指标影响较小,2组间差异均不显著(P>0.05)。由此可见,补饲10 g/d的RPC对成年黔北麻羊的采食量、瘤胃发酵参数和血浆生化指标没有显著影响。
The aim of this study was to investigate the effect of rumen-in-choline (RPC) on rumen fermentation and plasma biochemical parameters in adult northern Guizhou sheep and goats. Six adult Guizhou Qianghua sheep with good body condition, age and weight [(35.0 ± 1.2) kg] were divided into control group and experimental group with 3 replicates in each group and 1 sheep in each replicate. In a crossover design, the control group was fed basal diet without RPC; the experimental group was fed with 10 g / d RPC. The test lasted for 30 days, divided into two periods and each period was 15 days. The rumen fluid collected at the last day of each period was used to determine the concentrations of p H, ammonia nitrogen, microbial protein, volatile fatty acids (acetic acid, propionic acid and butyric acid) (Microcrystalline cellulose, carboxymethyl cellulose, cellobiase, xylanase) activity, while blood sampling determination of plasma glucose, urea nitrogen, total protein, albumin, total cholesterol, triglycerides, Creatinine concentration. The results showed that: 1) RPC had no effect on goat feed rate and had no significant effect on feed intake (P> 0.05); 2) RPC had no significant effect on p H, ammonia nitrogen concentration, microbial protein yield, volatile fatty acid concentration There was no significant difference between the two groups (P> 0.05). (3) RPC had little effect on the biochemical indexes of plasma, but there was no significant difference between the two groups (P> 0.05). Thus, supplementation of 10 g / d RPC had no significant effect on feed intake, rumen fermentation parameters and plasma biochemical parameters in adult Guizhou north sheep.