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目的探讨徐州地区感染性腹泻病人常见病毒组成,为病毒性腹泻的防治提供科学依据。方法采集2012年3月至2013年2月全市3个地区7家哨点医院急性腹泻患者的271份粪便标本,采用Real-Time PCR方法检测轮状病毒、诺如病毒、札如病毒、腺病毒和星状病毒。结果检测的271份标本,5种病毒的阳性率为20.66%,其中轮状病毒2.58%、诺如病毒8.49%、札如病毒0.74%、腺病毒1.11%,星状病毒9.96%。5种病毒主要是感染15岁以下(0~4岁组阳性率35.29%,5~14岁组阳性率33.33%);且有明显的季节特征,发病高峰在每年10月、11月和12月为主。结论星状病毒是导致徐州地区病毒性腹泻的主要病原,其次为诺如病毒。并存在病毒混合感染的情况。
Objective To investigate the composition of common viruses in patients with infectious diarrhea in Xuzhou and provide a scientific basis for the prevention and treatment of viral diarrhea. Methods A total of 271 stool specimens from acute diarrhea were collected from 7 sentinel hospitals in three districts of the city from March 2012 to February 2013. Real-time PCR was used to detect rotavirus, Norovirus, Sapporo virus, adenovirus And astrovirus. Results The positive rate of the five viruses was 20.66% in 271 samples tested. Among them, rotavirus 2.58%, Norovirus 8.49%, Sapporo virus 0.74%, Adenovirus 1. 9% and Astrovirus 9.96%. The five viruses were mainly infected under the age of 15 (the positive rate was 35.29% in 0-4 years old group and 33.33% in 5-14 years old group), with obvious seasonal features. The peak incidence peak was in October, November and December each year Mainly. Conclusion Astrovirus is the main pathogen causing viral diarrhea in Xuzhou, followed by Norovirus. And the existence of mixed virus infection situation.