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【目的】分析比较新生儿感染性疾病(败血症与一般感染)血浆中部分细胞因子的变化特征。【方法】采用双抗体夹心酶联免疫吸附实验法(ELISA)测定白细胞介素6(interleukin,IL-6)、肿瘤坏死因子α(tumer necrosisfactor,TNF-α)的水平,使用硝酸还原酶法测定NO水平。【结果】败血症患儿血浆IL-6、TNF-α、NO水平显著高于健康对照组(P<0.01),一般感染患儿血浆IL-6水平与健康对照组相比,差异无统计学意义(P>0.05)。新生儿缺血缺氧性脑病(hypoxic-ischemic encephalopathy,HIE)合并感染组TNF-α较单纯感染组差异无统计学意义(P>0.05),IL-6水平降低(P<0.01),而NO水平显著增高(P<0.01)。【结论】新生儿血浆中高水平的IL-6、TNF-α、NO提示机体存在感染,TNF-α可做为早期诊断的灵敏指标,并与感染轻重程度有关。新生儿窒息缺氧可影响IL-6和NO的水平。
【Objective】 To analyze and compare the changes of some cytokines in neonates with infectious diseases (sepsis and general infection). 【Methods】 The levels of interleukin (IL-6) and tumor necrosis factor α (TNF-α) were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) NO level. 【Results】 The levels of plasma IL-6, TNF-α and NO in sepsis children were significantly higher than those in healthy controls (P <0.01). There was no significant difference in plasma IL-6 levels between children with general infection and healthy controls (P> 0.05). Compared with the simple infection group, the TNF-αlevels in neonates with hypoxic-ischemic encephalopathy (HIE) infection had no significant difference (P> 0.05) and IL-6 levels decreased (P <0.01) The level was significantly higher (P <0.01). 【Conclusion】 High levels of IL-6, TNF-α and NO in the plasma of neonates indicate that there is infection in the body. TNF-α can be used as a sensitive indicator of early diagnosis, which is related to the severity of infection. Neonatal asphyxia hypoxia can affect the level of IL-6 and NO.