论文部分内容阅读
Ｈｅｐ－２细胞是脊髓灰质炎（脊灰）病毒的敏感细胞之一，其敏感性受多种因素制约，本文以培养Ｈｅｐ－２细胞是否使用乳蛋白水解物及小牛血清的质量差异对脊灰糖丸疫苗滴度的影响进行了分析。结果表明：乳蛋白水解物的使用可使脊灰三价糖丸疫苗总滴度平均提高０．３６ＬｏｇＣＣＩＤ５０／粒，显著高于未使用乳蛋白水解物培养的Ｈｅｐ－２细胞。另外，小中血清的影响不容忽视，本文发现有些进口小牛血清对病毒的生长产生非特异性抑制，对脊灰病毒的抑制滴度可达１．９ＬｏｇＣＣＩＤ５０／ｍｌ以上。这提示我们。组织培养试剂的更换应格外慎重，实验室标准体系的建立也是非常必要的。
Hep-2 cells are one of the sensitive cells of poliomyelitis (poliovirus). The sensitivity of Hep-2 cells is restricted by many factors. In this paper, whether Hep-2 cells cultured with milk protein hydrolyzate and calf serum The impact of the gray sugar pill vaccine titer was analyzed. The results showed that the use of milk protein hydrolyzate could increase the total titer of polio vaccine by 0.36LogCCID50 / grain, significantly higher than that of Hep-2 cells cultured without using milk protein hydrolyzate. In addition, the impact of small and medium serum can not be ignored, this paper found that some imported calf serum non-specific inhibition of the growth of the virus, inhibition of poliovirus up to 1.9LogCCID50 / ml or more. This prompts us. Tissue culture reagent replacement should be extra careful, the establishment of laboratory standards system is also very necessary.