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Rapid electron transfer from electron adduct and reducing OH adduct of 2’-deoxyadenosine-5’-monophosphate acid (dAMP) to riboflavin (RF) and flavin adenine dinudeotide (FAD) was studied using the time-resolved pulse radiolysLs techniques. Both spectroscopic and kinetic analyses showed that transient absorption spectrum of electron adduct or OH adduct of dAMP formed at first, and then changed to that of radical anion of RF or FAD after several microseconds of pulse. The evidence indicated that electron transfer from electron adduct and reducing OH adduct of dAMP to RF or FAD did occur. From buildup or bleaching kinetics of radical anions of RF and FAD, the rate constants for electron transfer were determined, respectively.
Rapid electron transfer from electron adduct and reducing OH adduct of 2’-deoxyadenosine-5’-monophosphate acid (dAMP) to riboflavin (RF) and flavin adenine dinudeotide (FAD) was studied using the time-resolved pulse radiolysLs techniques. Both spectroscopic and kinetic analyzes showed that transient absorption spectrum of electron adduct or OH adduct of dAMP formed at first, and then changed to that of radical anion of RF or FAD after several microseconds of pulse. The evidence indicated that electron transfer from electron adduct and reducing OH adduct of dAMP to RF or FAD did occur. From buildup or bleaching kinetics of radical anions of RF and FAD, the rate constants for electron transfer were determined, respectively.