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1972年,Halliday和Miller首先提出应用白细胞粘附抑制试验(LAI) 作为检测动物细胞免疫的方法。其后,由Holan,Grosser和Thomson等不断改进。近几年来,国外主要用来检测恶性黑色素瘤、乳癌、消化道肿瘤等。由于方法简便,有一定的敏感性和特异性,故临床应用逐渐增多,国内也相继开展了这方面的工作。现将我们初步应用的结果报道如下。材料与方法〔细胞的采集〕国外采用密度梯度法从静脉血分离白细胞。我们采用斑蝥酊发泡,从皮泡液内获取细胞的方法。即取斑蝥虫10克,加95％乙醇100毫升,置室温下浸泡两周,配成10％斑螫酊剂。试验时,用小滤纸片(1cm～2) 占湿斑蝥酊后,置于一侧前臂皮肤上,然后用一块有机玻璃片(2cm～2) 盖在滤纸片上,再在盖片上加放一块纱布,用胶布固定。4-5小时后,揭去纱布,盖片与滤纸,可见局部皮肤发红,表面开始松动。在此局部皮肤上加盖5×5cm大小的
In 1972, Halliday and Miller first proposed the use of leukocyte adhesion inhibition assay (LAI) as a method for detecting animal cell immunity. Since then, Holan, Grosser and Thomson have continued to improve. In recent years, foreign countries mainly used to detect malignant melanoma, breast cancer, gastrointestinal cancer and so on. Because of its simplicity and sensitivity and specificity, clinical applications have gradually increased and domestic efforts have been carried out in this area. The results of our preliminary application are reported below. Materials and Methods [Collection of Cells] The density gradient method was used to separate white blood cells from venous blood. We use canthalycin to obtain cells from the vesicular fluid. That is to take 10 grams of spot roundworm, plus 95% ethanol 100 ml, set for two weeks at room temperature, dubbed 10% spotting agent. During the test, use a small piece of filter paper (1cm to 2) to cover the wet spot, place it on the skin of one forearm, then use a piece of plexiglass sheet (2cm~2) to cover the filter paper, and put a piece of gauze on the cover piece. , fixed with tape. After 4-5 hours, the gauze, cover sheet and filter paper were removed, and the redness of the local skin was observed, and the surface began to loosen. Cover this area with 5×5cm