在细菌的保藏方法中 ,最为理想的方法即为低温真空干燥法 ,基本原理是运用干燥、低温和隔绝空气的手段 ,降低细菌新陈代谢的速度 ,使细菌的生命活动处于半永久性休眠状态 ,以达到长期保藏的目的[2 ] 。而此项技术设备条件要求高 ,一般实验室难以达到。因此 ,我们试验超低温保存 In the preservation of bacteria, the most ideal method is the low-temperature vacuum drying method, the basic principle is the use of dry, low temperature and isolated air means to reduce the speed of bacterial metabolism, so that the activities of bacteria in the semi-permanent dormancy in order to achieve The purpose of long-term preservation [2]. The high technical equipment requirements, the general laboratory is difficult to achieve. Therefore, we tested cryopreservation