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ＢＡＬＢ／Ｃ小鼠经腹部接种Ｂ１６黑色素瘤细胞建立荷瘤鼠模型，经静脉注射吐温８０及腹部４１℃温热，于处理后１周和２周测定肿瘤坏死因子（ＳｅｒｕｍＴｕｍｏｒＮｅｅｒｏｓｉｓＦａｅｔｏｒ，ＳＴＮＦ）活性及血清唾液酸含量；并观察作用后荷瘤鼠死亡率。结果显示：荷瘤鼠ＳＴＮＦ活性及唾液酸含量持续保持相对高水平（正常ＢＡＬＢ／Ｃ鼠ＳＴＮＰ活性检测不出；唾液酸含量＜５０ｍｇ／ｍｌ）。单独以吐温８０处理和吐温８０合并４１℃温热处理的荷瘤鼠ＳＴＮＦ活性显著降低（Ｐ＜０．０５；Ｐ＜０．０１）。同时吐温８０合并４１℃温热可显著降低荷瘤鼠死亡率（Ｐ＜０．０５）。以上表明：吐温８０尤其吐温８０合并４１℃温热可通过影响荷瘤鼠ＳＴＮＰ，发挥其一定的抗肿瘤免疫作用。
A tumor-bearing mouse model was established in BALB/C mice by inoculation of B16 melanoma cells in the abdomen. Tween 80 was injected intravenously and warmed at 41°C in the abdomen. Serum and tumor necrosis factor (STNF) activity was measured at 1 and 2 weeks after treatment. Serum sialic acid content; and observe the mortality of tumor-bearing mice. The results showed that the activity of STNF and the content of sialic acid in tumor-bearing mice continued to maintain a relatively high level (no detectable STNP activity in normal BALB/C mice; sialic acid content <50 mg/ml). The STNF activity of tumor-bearing mice treated with Tween 80 alone and Tween 80 combined with 41°C heat treatment was significantly reduced (P<0.05; P<0.01). At the same time, Tween 80 combined with 41 °C warming can significantly reduce the mortality of tumor-bearing mice (P <0.05). The above shows that: Tween 80, especially Tween 80 combined with 41 °C warming can affect the tumor-bearing mice STNP, play a role in its anti-tumor immunity.