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目的探讨三碘甲腺原氨酸(T_3)对大鼠成骨细胞表达碱性磷酸酶(ALP)、骨钙素(OC)的影响。方法采用骨组织块法原代分离培养新生SD大鼠颅骨成骨细胞,然后配置含不同浓度(10~(-9)、10~(-8)、10~(-7) mol/L)T_3的培养基,与大鼠成骨细胞共培养4 d,采用比色法测定细胞ALP,采用放射免疫法测定成骨细胞OC表达变化。结果T_3可促进大鼠成骨表达ALP,随T_3浓度的增高,成骨细胞表达ALP活性增强(F=17.60,P=0.039);10~(-8)、10~(-7) mol/L T_3促进成骨细胞表达OC(F=19.25,P=0.032)。结论 T_3可以刺激成骨细胞表达ALP、OC。
Objective To investigate the effects of triiodothyronine (T_3) on the expression of alkaline phosphatase (ALP) and osteocalcin (OC) in rat osteoblasts. Methods Osteoblasts of neonatal SD rats were primarily isolated and cultured by using the method of bone tissue block. Then the cells were cultured in different concentrations (10 -9, 10 -8, 10 -7 mol / L) T 3 Cultured with rat osteoblasts for 4 days. ALP was measured by colorimetric assay. The OC expression of osteoblasts was measured by radioimmunoassay. Results T_3 promoted the osteoblast expression of ALP. With the increase of T_3 concentration, the ALP activity of osteoblasts increased (F = 17.60, P = 0.039) T_3 promoted osteoblast expression of OC (F = 19.25, P = 0.032). Conclusion T 3 can stimulate osteoblasts to express ALP and OC.