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目的:探讨抗肿瘤坏死因子-α单克隆抗体(TNF-αmAb)对肝肺综合征(HPS)大鼠的治疗作用。方法:采用胆总管结扎(CBDL)建立大鼠HPS模型,将60只雄性SD大鼠随机分为假手术组、模型组和干预组。模型组:分别于结扎后1、2、3、4及5周麻醉动物(每个时间点6只大鼠);干预组:于结扎后第2周,开始腹腔注射抗TNF-αmAb(0.1mg/kg.2d),干预后1、2、3及4周麻醉动物(每个时间点6只大鼠);假手术组(6只大鼠)与5周模型组动物同批麻醉,留取各组动物的肝组织,分别行HE和Masson染色后,观察肝组织病理变化。抽取动脉血进行血气分析,计算肺泡、动脉氧分压差的变化。抽取各组动物的静脉血并分离血清,检测肝功能、内毒素、TNF-α、NO的水平。结果:干预组肝组织的炎症和纤维化明显减轻,动脉血气分析显示干预组动脉-肺泡氧分压差明显减少(P<0.05),肝功能、内毒素、TNF-α及NO的水平较模型组明显好转(P<0.05)。结论:抗TNF-αmAb对HPS大鼠具有治疗作用。
Objective: To investigate the therapeutic effect of anti-tumor necrosis factor-α monoclonal antibody (TNF-α mAb) on hepatopulmonary syndrome (HPS) rats. Methods: The model of HPS was established by common bile duct ligation (CBDL). Sixty male Sprague-Dawley rats were randomly divided into sham operation group, model group and intervention group. Rats in model group were anesthetized 1, 2, 3, 4 and 5 weeks after ligation (6 rats at each time point). Intervention group: At the second week after ligation, anti-TNF-α mAb /kg.2d). Animals were anesthetized at 1, 2, 3 and 4 weeks after the intervention (6 rats at each time point), sham operation group (6 rats) The liver tissues of each group were stained with HE and Masson to observe the pathological changes of liver tissues. Arterial blood was drawn for blood gas analysis to calculate changes in alveolar and arterial oxygen partial pressure. The venous blood of each group of animals was drawn and the serum was separated to detect the levels of liver function, endotoxin, TNF-α and NO. Results: Inflammation and fibrosis in the intervention group were significantly reduced. Arterial blood gas analysis showed that the difference in arterial-alveolar oxygen pressure in the intervention group was significantly reduced (P <0.05), and liver function, endotoxin, TNF-α and NO levels were significantly lower than those in the model Group improved significantly (P <0.05). Conclusion: Anti-TNF-α mAb has a therapeutic effect on HPS rats.