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为了细致地观察肺内弹性蛋白的变化,作者采用较特异的电镜标本制备方法,观察了弹性酶肺气肿肺内弹性蛋白的超微结构变化及弹性酶肺气肿的发生、发展规律。作者采用的鞣酸和磷钨酸染色对于电镜下观察弹性蛋白的变化十分有利,其效果远远优于常规的醋酸铀—柠檬酸铅染色。观察结果表明,注弹性蛋白酶后3日,弹
In order to observe the changes of elastin in the lungs carefully, the author used a more specific method of electron microscopy to observe the ultrastructural changes of elastase and the occurrence and development of elastase emphysema. The tannin and phosphotungstic acid staining used by the authors is very beneficial for the observation of the change of elastin under electron microscopy, and its effect is much better than the conventional uranyl acetate-lead citrate staining. Observations show that, after injection of elastase on the 3rd, shells