论文部分内容阅读
通过对香菇菌丝体进行液体和固体富锌培养,以确定香菇菌丝体在外源锌的作用下生长和抗氧化酶活性的变化规律。香菇菌丝体具有较强的耐锌和富锌能力,低浓度锌对香菇菌丝体的生长有促进作用,高浓度有抑制作用,适宜的锌质量浓度为600 mg/L。在锌质量浓度400~1 000 mg/L,香菇菌丝体超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化物酶(Peroxidase,POD)和过氧化氢酶(Catalase,CAT)随锌浓度增加先升高再降低,最大值分别为:147.47 U/g、6.15×10~(-3)U/g和147.52 U/g;菌丝体丙二醛(Malondialdehyde,MDA)含量随锌浓度增加先呈下降的趋势,然后逐渐上升,在锌质量浓度600 mg/L时达到最低,为4.47×10~(-5)μmol/g。香菇菌丝体抗氧化酶系随培养时间变化规律的研究结果表明,香菇菌丝体在培养的第9天,SOD、POD和CAT的酶活性均达最大值,MDA含量达到最低值,分别为:154.31 U/g、6.21×10-3U/g、147.92 U/g和4.18×10~(-5)μmol/g。香菇菌丝体在适宜浓度富锌培养,不仅对香菇菌丝体的生长有促进作用,同时可提高香菇菌丝体的锌含量,并且提高了香菇菌丝体的抗氧化酶活性。
The lentinus mycelium liquid and solid zinc rich culture to determine the growth of mycelium of lentinan under the action of exogenous zinc and changes in antioxidant enzyme activity. Lentinus edodes mycelium has strong resistance to zinc and zinc-rich ability. Low concentration of zinc can promote the growth of Lentinula edodes mycelia, inhibit the high concentration of zinc, the suitable concentration of zinc is 600 mg / L. Under the condition of zinc concentration of 400-1 000 mg / L, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) The contents of MDA increased with the increasing of zinc concentration, and the maximum values were 147.47 U / g, 6.15 × 10 -3 U / g and 147.52 U / g, respectively. The content of malondialdehyde It showed a trend of descending firstly and then gradually increased, reaching the lowest level of 4.47 × 10 ~ (-5) μmol / g when the zinc concentration was 600 mg / L. The results showed that the activities of SOD, POD and CAT in the mycelium of Lentinula edodes reached the maximum value and the content of MDA reached the minimum : 154.31 U / g, 6.21 × 10-3 U / g, 147.92 U / g and 4.18 × 10 ~ (-5) μmol / g. Lentinus edodes mycelium in the appropriate concentration of zinc-rich culture, not only promote the growth of lentinan mycelium, but also improve the mycelium of lentinan zinc content, and increased mycelium antioxidant activity of mushroom.