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从1970年Mayes和Bagard等介绍血浆醛固酮的放射免疫测定以来,其它测定方法也陆续有所报道,但这些方法除对血浆样品用有机溶剂提取外,大多还需再进行一次纸层析、柱层析或薄板层析纯化,以去除其它甾体激素的干扰。虽有人用醛固酮-γ-酯-BSA作抗原或用免疫纯化法来提高特异性,但都较繁琐。我们在尿醛固酮放射免疫测定的基础上,进一步采用高比放射性的[1,2,6,7-~3H]醛固酮作标记抗原,同时改变实验系统的条件,建立不经层析的、高灵敏的血浆醛固酮放免测定方法。
Since the Mayes and Bagard in 1970 introduced plasma aldosterone radioimmunoassay since the determination of the other methods have also been reported, but these methods in addition to the plasma sample extracted with organic solvents, most of the need for further paper chromatography, the column Analysis or thin-layer chromatography to remove the interference of other steroid hormones. Although some people use aldosterone-gamma-ester-BSA as antigen or immunopurification to improve the specificity, they are more complicated. On the basis of urinary aldosterone radioimmunoassay, we further used highly-radioactive [1, 2,6,7-] 3H aldosterone as the labeled antigen and changed the conditions of the experimental system to establish a non-chromatographic, highly sensitive Plasma aldosterone radioimmunoassay method.