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组织化学是研究胆石化学构成的定位、定性和定量的可行方法之一,但是由于胆石脆而易碎,普通石蜡切片不能获得满意的胆石切片标本。中国医大戴显伟等用石蜡薄切片树脂增强技术得到了满意的组化切片标本。我们在此基础上加以改进,用石蜡假包埋4％火棉胶涂膜制作了可供组化研究的胆石切片,现将制作方法和组化染色过程报告如下。胆石切片的制作①浸蜡和假包埋:手术取出之胆石,自然干燥后,中央剖开,浸入58℃蜡液中,恒
Histochemistry is one of the feasible methods to study the localization, characterization and quantification of the chemical composition of gallstone. However, due to the brittleness and fragility of gallstone, ordinary paraffin sections can not obtain satisfactory gallstone specimens. China Medical University, Dai Xianwei and other thin-section paraffin wax resin reinforced technology has been satisfactorily organized specimens. On the basis of this, we make improvements, using paraffin wax embedding 4% collodion film made of galenic stones for the study of the organization, now the production methods and histochemical dyeing process is reported as follows. Gallstone slice production ① Baptist wax and false embedding: gallstone removed by surgery, natural drying, the central cut open, immersed in 58 ℃ wax solution, constant