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目的 采用测定胆汁中癌胚抗原 (CEA)含量并结合蛋白质印迹 (Westernblot)技术排除CEA相关物质的干扰 ,提高CEA检测胆道恶性肿瘤的特异性。方法 对临床可疑为胆道恶性肿瘤的患者采用酶联免疫吸附 (ELISA)法测定其血液、胆汁中CEA含量 ,对胆汁CEA含量升高的患者进一步应用Westernblot半干转移技术区分CEA及CEA相关物质。结果 所有CEA含量升高的胆道恶性肿瘤患者的胆汁经Westernblot检测均表现为包含有一条相对分子质量为 2 10 0 0 0的特异条带 ,而胆道良性疾病患者既使其CEA含量高于正常也均不含有该条带。结论 采用Westernblot技术检测胆汁CEA可排除CEA相关物质的干扰 ,提高CEA检测胆道恶性肿瘤的特异性。
OBJECTIVE: To determine the specificity of CEA in the detection of malignant tumors of the biliary tract by measuring the content of carcinoembryonic antigen (CEA) in bile and removing the interference of CEA-related substances by Western blotting. Methods CEA levels in blood and bile were determined by enzyme-linked immunosorbent assay (ELISA) in patients with clinically suspected biliary malignancies. CEA and CEA-related substances were further identified by Western blot semi-dry transfer in patients with elevated CEA levels. Results All patients with biliary malignancy with elevated CEA levels showed a specific band with a molecular mass of 210,000 by Western blot analysis. However, patients with benign biliary disease had higher CEA levels than normal Do not contain the strip. Conclusion Detection of bile CEA by Western blotting can eliminate the interference of CEA-related substances and improve the specificity of CEA in the detection of biliary malignant tumors.