论文部分内容阅读
为分析Ｄ２Ｓ４４１、Ｄ２Ｓ１１９和Ｄ２Ｓ１２３３个微卫星ＤＮＡ标记位点不稳定性，研究ＳＮＰＣＣ发生机理的分子基础。应用ＰＣＲ－ＳＳＬＰ扩增微卫星ＤＮＡ，聚丙烯酰胺凝胶电泳方法对３１例临床诊断为散发性非息肉性大肠癌的肿瘤组织及其相应的正常组织的Ｄ２Ｓ４４１、Ｄ２Ｓ１１９和Ｄ２Ｓ１２３３个微卫星ＤＮＡ标记位点进行分析。结果显示，３１例中，２例在所检测的３个位点均存在微卫星ＤＮＡ不稳定性。提示微卫星ＤＮＡ不稳定性与散发性非息肉性大肠癌发生有关，可通过检测微卫星ＤＮＡ不稳定性筛选大肠癌高危人群，以便早期预防。
In order to analyze the instability of D2S441, D2S119 and D2S1233 microsatellite markers, the molecular basis of the mechanism of SNPCC was studied. PCR-SSLP was used to amplify microsatellite DNA and polyacrylamide gel electrophoresis was used to detect the D2S441, D2S119 and D2S1233 microsatellite DNA markers in 31 cases of sporadic non-polyposis colorectal cancer. Site analysis. The results showed that there were microsatellite DNA instability in 3 of the 31 sites tested. It suggests that microsatellite DNA instability is related to the occurrence of sporadic nonpolyposis colorectal cancer. Screening for high-risk populations of colorectal cancer can be performed by detecting microsatellite DNA instability for early prevention.