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本实验用微玻管将50％HRP 0.02微升注入12例大白鼠中缝大核,从颈髓到骶髓均可见到标记细胞,腰段较多;分布于双侧Ⅱ板层至Ⅹ板层,以Ⅶ板层为最多,其次为Ⅴ板层,Ⅳ板层居第三位,总的分布为Ⅶ层>Ⅴ层>Ⅳ层>Ⅲ层>Ⅷ层>Ⅹ层>Ⅵ层>Ⅸ层>Ⅱ层。标记细胞形态有圆形、卵圆形、多角形及梭形等。多数在20—40微米之间,占标记细胞总数的61％,大于40微米者占12％,小于20微米者占27％。本实验证明脊髓灰质向中缝大核的投射较为广泛,为中缝大核针刺镇痛的机理提供了形态学依据,同时讨论了脊髓灰质—中缝大核纤维联系的生理意义。
In this experiment, 50 microliters of HRP was injected into 12 microglial mesenchymal nuclei with 50 microliters of HRP, and labeled cells were seen from the cervical cord to the sacral sac with more lumbar segments. They were distributed in the lamina Ⅱ to the laminae Ⅹ , With the highest in Ⅶ plate, the next in Ⅴ plate, the third in plate Ⅳ, with the total Ⅶ> Ⅴ> Ⅳ> ⅲ> Ⅷ> Ⅹ> Ⅵ> Ⅸ> Ⅱ layer. Marked cell morphology round, oval, polygonal and fusiform and so on. Most of 20-40 microns, accounting for 61% of the total number of labeled cells, more than 40 microns accounted for 12%, less than 20 microns accounted for 27%. This experiment demonstrated that the projection of spinal cord gray matter to the large nucleus of the stitches is more extensive, which provides a morphological basis for the mechanism of acupuncture analgesia of the large stitches and discusses the physiological significance of the connections between the gray matter and the macroscopical fibers.